| The Possible Protective Effect of Nifedipine Against L-Arginine Induced Acute Pancreatitis in Adult Male Albino Rats: Histological and Immunohistochemical Study |
| Paper ID : 1004-HISTO23 (R2) |
| Authors |
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Aya Taha Abd elmwgood * Faculty of medicine, Histology department, BSU |
| Abstract |
| Background: Acute pancreatitis (AP) is an inflammatory disease, with high morbidity and mortality rates. Oxidative stress plays an important role in pancreatic-acinar cell injury in AP. Nifedipine is a calcium channel blocker, widely used in treatment of hypertension, angina, congestive heart failure and had been proved to show antioxidanantiinflammatory properties. Aim of work: to investigate the possible protective effect of Nifedipine against L-arginine induced AP in adult male albino rats. Material and methods: Forty-adult male albino rats were divided into five groups (n=8/group) comprising of normal control group, Nifedipine group (5mg/Kg/p.o/1week) , L-arginine group: AP was induced by two intraperitoneal injections of L-arginine Hcl (250mg/100g) with 1hour interval between the two doses, L-arginine + Nifedipine group at the above mentioned doses, Recovery group (two intraperitoneal injections of L-arginine Hcl at dose of (250 mg/100gm) with 1hour interval between the two doses. After 1week for all groups and 2weeks for recovery group, rats were sacrificed, body weights were recorded, blood samples were taken, and their pancreas were biochemically, histologically, immuno-histochemically, and morphometrically studied. Results: AP revealed distorted pancreatic acini, dilated and congested blood vessels, and inflammatory cell infiltration, which were reversed using Nifedipine. Nifedipine reduced serum amylase and lipase levels, oxidative stress (MDA, MPO, TNF-α), and increased GSH and Nrf2 levels in pancreatic tissue. Furthermore, it reduced Caspase 3 and NF-kB immunoreactivity. Histological and biochemical findings improved slightly in the recovery group without any treatment. Conclusions: Nifedipine treated AP. Thus, AP adjuvant treatment with Nifedipine is advised. |
| Keywords |
| Acute Pancreatitis, Nifedipine, L-Arginine, oxidative stress, NF-kB, Caspase 3. |
| Status: Abstract Accepted (Poster Presentation) |