| Histological study of the Role of Mesenchymal stem cell derived exosomes versus melatonin on Skin wound healing of Adult Male Albino Rats |
| Paper ID : 1005-HISTO23 (R1) |
| Authors |
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Amany Mohammed El Saeed Abd El Hamid *1, Dalia Hussein Abd El Aziz2, Samraa Hussein Abdelkawi3, Fatma El Zahraa Mohammed Abd El Latif4 1Faculty of medicine,Beni Seuf University 2Faculty of medicine,Beni seuf university 3Faculty of medicine banisuef university 4Faculty of Medicine-Beni-Suef University |
| Abstract |
| Background: Wound healing involves interaction of growth factors and cytokines to restores tissue integrity. Melatonin and MSC-derived exosomes have anti-inflammatory effect, enhance re-epithelization, angiogenesis and regulate collagen remodeling. Aim of work: to evaluate effect of melatonin versus MSC-derived exosomes in skin wound healing. Materials and Methods: 28 male albino rats were divided equally into 4 groups: Group I (control) left unwounded. Other groups, full-thickness dermal circular wounds were created. Group II, wound left untreated. Group III (melatonin-treated), melatonin was dissolved in saline and given orally in a dose of 5 mg/kg once daily for 14 days. Group IV (exosomes-treated), exosomes were injected once subcutaneously at four sites around wound at a dose of 200 μg MSC-exosomes in 200 μl PBS. After 14 days, skin sections were examined histologically (by light and electron microscopes). Results: Histological and ultrastructural examination of group I was similar to normal skin histological structure. H&E stained sections of group II revealed loss of epidermal continuity at the site of wound with massive inflammatory cells infiltration and angiogensis, while masson stained sections showed thin disorganized collagen fibers in dermis. EM examination of skin sections of group II showed loss the hemidesmosomal junction with basement membrane damage and loss of cell to cell junction. Both melatonin and MSC-derived exosomes succeeded in restoration of normal histological structure of skin at wound site with better results in exosomes treated group. Conclusion: melatonin and MSC-derived exosomes have ameliorative effect in skin wound healing. |
| Keywords |
| Key words: skin, melatonin, exosomes. |
| Status: Abstract Accepted (Oral Presentation) |