| Oral supplementation of spirulina platensis alleviates methotrexate-cerebellar neurotoxicity in male rats: Histological and Biochemical studies |
| Paper ID : 1007-HISTO23 (R1) |
| Authors |
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Asmaa Hamed AbdElrahman *1, Eman Hosny Kandil2, Hani Abdel Hamed Abdel Samie3, Amany Ebrahim Nofal4 1معيده في كلية العلوم جامعه المنوفيه ، قسم علم الحيوان شعبة الخلايا والأنسجه 2Lecturer of Cytology, Histology and Histochemistry, Zoology Department, Faculty of Science, Menoufia University. 3Professor of Histology and Histochemistry, Zoology Department, Faculty of Science, Menoufia University 4Associate Professor of Histology and Histochemistry, Zoology Department, Faculty of Science, Menoufia University, 32511, Shebin El- Kom, Menoufia, Egypt |
| Abstract |
| Abstract Introduction: Methotrexate (MTX), a drug utilized in cancer and rheumatoid arthritis treatment is associated with acute and chronic neurodegenerative alterations. Objectives: The current study investigates the possible effect exerted by the blue green algae Spirulina platensis (SP) against neurotoxicity of methotrexate in male rats. Material and Methods: Forty adult male Wistar rats were divided into four groups (n=10) and treated for one week: G1 was the control group, G2 was orally given 1000 mg Spirulina/kg/daily for one week ,G3 was given a single intraperitoneal injection of 75mg methotrexate/kg on the 2nd day, and G4 was given both Spirulina and Methotrexate with the same previous doses and duration. Results: Methotrexate caused severe neurodegeneration in the 3 cerebellar cortex layers specially Purkinje layer. Such alterations include disturbed cerebellar architecture, vascular dilatation and cytoplasmic vacuolization. Purkinje layer displays disturbed monolayer arrangement with pyknotic nuclei, significant cell number decrease, shrunken cells surrounded by empty spaces. The molecular and granular layers had dispersed cells with prominent perineuronal spaces with elevated immunoreaction with GFAP, IBA-1 and NFL. Moreover, methotrexate significantly increased malondialdehyde and myeloperoxidase, while decreased level of glutathione, serotonin, superoxide dismutase, acetylcholinesterase, norepinephrine and dopamine. These insults were noticeably mitigated by concomitant treatment with spirulina. Conclusion: Spirulina offers neurological protective role in modulating the cerebellar neurotoxicity elicited by MTX. Such neuroprotection may be attributed to its antioxidant and anti-inflammatory properties. Interestingly, supplementation could be a new way in decreasing the nervous system complications due to chemotherapy. |
| Keywords |
| Keywords: Cerebellum, Methotrexate, Spirulina, histology, ultrastructure, immunohistochemistry, biochemistry. |
| Status: Abstract Accepted (Oral Presentation) |