| Potential Effect of the Phenobarbital in Albino Mice liver; Biochemical and Immunohistochemical Studies |
| Paper ID : 1009-HISTO23 (R1) |
| Authors |
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Mona AH Yehia *, Hagar M Youssef Alexandria University - Medical Research Institute |
| Abstract |
| Background : Phenobarbital (PB) is the oldest and important antiepileptic drug in common use of many countries. Objective: This work aimed to determine the possible effect of PB in the albino mice liver using histochemical and biochemical studies. Material and Method: Forty albino mice (one week age) were divided into equal four groups (10 mice each); control group and three groups were fed diet with PB at doses of 1, 7.5 and 15 mg/kg body weight for six weeks. The blood serum was collected and frozen to study the biochemical liver enzymes. Liver specimens were prepared for histological, histochemical (γ-glutamyl transpeptidase enzyme (GGT)) and immunohistochemical (placental glutathione S transferase enzyme (GST-P)) studies. Results: Serum ALP, ALT, GGT and GST-P were gradually increased with raised PB dose. Histological finding in liver sections revealed focal areas of hepatocytes with sign of preneoplastic changes were advanced with increased PB doses. In the cytoplasm of the hepatocytes, GGT enzyme deposit was appeared as diffuse brown staining and immunostaining expression of GST-P enzyme showed small brown granules, both gradually increased proportional to the dose intake. Conclusion: It was proved that serum ALP, ALT, GGT, and GST-P increased, as well as, histochemical staining of GGT and immunohistochemical staining of GST-P in liver tissue were increased. This elevation of liver enzymes clarifies hepatic injury during PB intake. Therefore, PB can be considered a promoter of hepatic injury and GGT and GST-P enzymes have the potential as reliable markers of early diagnostic the liver injury. |
| Keywords |
| Mice liver, liver enzymes, γ-glutamyl transpeptidase enzyme (GGT), placental glutathione S transferase enzyme(GST-P), Phenobarbital. |
| Status: Abstract Accepted (Poster Presentation) |